Dorsal vs ventral blastoporal lip3/20/2024 ![]() We observed, however, time differences in the expression pattern. The expression of Lim1 in embryos of these frogs was comparable with the Xenopus laevis expression pattern. Thesefrogs belong into four separate families, and have differences in their modes of reproduction, and developmental rates. Embryos of four different frogs were analyzed, besides Xenopus laevis, we studied embryos of the foam-nesting frog, the poison-arrow frog, andof the marsupial frog. Masanori Taira and Norihiro Sudou at the University of Tokyo, Japan cross reacted with embryos of the species tested, and gave a specific nuclear signal, which was expected, as the protein Lim1 is a transcription factor. This antibody, which was developed by our co authors Drs. The antibody was raised against the conserved C terminal region of Xenopus laevis Lim1 protein. To analyze the expression of Lim1 in different frogs we produced a polyclonal antibody against the protein Lim1. Its ectopic expression in the ventral blastopore lip of the Xenopus laevis gastrula produces duplication of the dorsal axis. One of the organizer genes is Xlim1 (also known as Xenopus Lhx1), a highly conserved gene among vertebrates. The dorsal blastopore lip of amphibian embryos, which is the Spemann–Mangold organizer, expresses specific genes,and initiates the development of dorsal axial structures. In what follows, I will introduce our article entitled “Comparison of Lim1 expression in embryos of frogs with different modes of reproduction”, published in the “International Journal of Developmental Biology” in 2009. In our laboratory at the School of Biological Sciences of the Pontifical Catholic University of Ecuador, in Quito Ecuador we study the developmental strategies of tropical frogs in comparison with the frog Xenopus laevis to gain insights into the mechanisms of early development. Michael Venegas-Ferrín, Norihiro Sudou, Masanori Taira and Eugenia M. Comparison of Lim1 expression in embryos of frogs with different modes of reproduction
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